A Simple Method to Prepare Affinity Resins on Cellulose Basis

Affinity resins with different spacer arms were synthesized analogues to the solid phase peptide synthesis using aminoethyl cellulose or carboxymethyl cellulose as matrix. The spacer arms could be varied in length and rigidity. Especially spacer arms consisting o f poly amino acids can be synthesized with a defined amount o f amino acid residues specifically in the low molecular weight ranges. The method is also applicable to other matrices which are not susceptible to 1 N HC1 in glacial acetic acid, trethylamine, methylene chloride and dim ethylform am ide. The synthesis o f affinity resins with different spacer arms for the purification o f androgen receptors is described as an example o f the method.